Fluorescence polarization immunoassay wikipedia
How can the answer be improved?Fluorescence polarization immunoassay Fluorescence polarization immunoassay (FPIA) is a class of in vitro biochemical test used for rapid detection of antibody or antigen in sample. FPIA is a competitive homogenous assay, that consists of a simple prepare and read method, without the requirement of separation or washing steps. fluorescence polarization immunoassay wikipedia
Fluorescence Polarization Immunoassay FPIA FPIA Fluoresceinlabelled drug competes with unlabelled drug for antibody Sample excited with plane polarized light (490 nm) Fluorescein emits plane polarized light (520 nm) Small, free drugfluorescein, rotates faster leading to less emission
Fluorescence polarization or fluorescence anisotropy (FPFA) is a versatile solutionbased technique that has been widely used to study molecular interactions, enzymatic activity, and Fluorescence polarization immunoassay (FPIA) differs from ELISA in that it is a homogeneous assay conducted in solution phase. Unlike heterogeneous immunoassays, homogeneous assays do not require the separation of the free and bound tracer.fluorescence polarization immunoassay wikipedia Proficiencytesting data from the College of American Pathologists for 2010 reveal that 92 of laboratories perform the fluorescence polarization immunoassay used in this case, and all of the 407 participating laboratories currently use an immunoassay platform for MTX.